![]() In a recent clinical application study, the assay also resulted in improved prescription of antimicrobial therapy, a reduction in isolation days of admitted patients, and detection of pathogens that were not requested to investigate by the clinician. The GenMark Respiratory Pathogen Panel (RPP) assay on the ePlex instrument was evaluated in several clinical studies and showed excellent overall agreement of over 95 % compared to laboratory-developed (multiplex) real-time PCR assays (LDTs) in samples with cycle threshold (C T) values < 35. These assays are able to rapidly detect multiple pathogens associated to clinical syndromes, including viruses, bacteria and parasites. There is substantial progress in the development of syndromic testing platforms for respiratory infections, gastroenteritis and even neurological infections. ![]() Some bacterial pathogens, like Legionella pneumophila, are critical to detect because they represent important epidemiologic challenges and can cause serious complications that require treatment strategies different from standard empiric regimens. The clinical performance of both assays depended highly on the bacterial load in the sample and the type of specimen under investigation.Ĭommunity-acquired respiratory tract infections are a leading cause of hospitalization worldwide and a significant cause of mortality, especially in vulnerable patient groups. No false-positive results were reported for all three bacterial pathogens by both assays. pertussis positive samples and 13/15 (86.7 %) of the M. ![]() pneumophila targets, 8/12 (66.7 %) of the B. The ePlex Respiratory Pathogen Panel (RPP) assay detected 10/14 (71.4 %) of the L. pertussis positive samples but only 11/15 (73.3 %) of the M. The QIAstat-Dx Respiratory Panel V2 (RP) assay detected all of the L. Fifty-six specimens were collected from our repositories, five negative samples and fifty-one samples which had been previously tested positive with the routine diagnostic real-time PCR assays for Legionella spp. Aim of this study was to evaluate the performance of these syndromic panels for these three bacterial targets in samples from the LRT. For diagnosing bacterial pneumonia, lower respiratory tract (LRT) specimens are indicated. The assays have been marketed for use in nasopharyngeal swab specimens. The ePlex® and QIAstat-Dx® respiratory pathogen panels detect multiple respiratory pathogens, mainly viruses but also Legionella pneumophila, Mycoplasma pneumoniae and Bordetella pertussis.
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